Deletion

Deletion

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Deletion ID	Start	Band	Stop	Band	Element1	Element2	Chr	Deletion (bp)	Add
Df(2L)ED494	7423266	27F4	7576637	28B1	5-HA-1178	UM-8100-3	2L	153371	-

Add components to make deletion

Made	Yes	Available	Yes	Made By	drosdel
PCR Confirmed	No	Genetic Confirmed	No	Attempted and failed?	No
Orientation	forward
Comments

Confirmation primers. Pair each primer with the PRY4 primer for PCR.

Primer	Sequence	Tm	GC%	Product (bp)
494.A	TTGAAAAACCTAAGAATCGACCA	59.99	34.78	217
494.B	TCTTTGTCTGCGCTGCAT	59.23	50.00	230

deletes the following gene(s)

Name	Start	Stop	Name	Start	Stop	Name	Start	Stop
CR33583	7425853	7425875	CR33608	7425979	7426001	CG5261	7426866	7430329
CG5958	7430529	7433480	CG5973	7437506	7445269	CG12789	7445345	7451165
CG13787	7452525	7454169	CG13788	7455008	7462247	CG6055	7466550	7472205
CG15819	7472505	7474893	CG6441	7474702	7476750	CG6630	7476882	7483823
CG6641	7496867	7497360	CG33529	7497805	7544423	CG13790	7568534	7568818

partially deletes the following gene(s)

Name	Start	Stop